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HISTOCHEMICAL STUDY ON THE ORAL MUCOSA OF METHIONINE ADMINISTERED RATS

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Abstract


The buccal mucosa was observed histochemically in male rats which were injected with methionine.
The staining methods were proceed by means of periodicacid-Schiff reaction, toluidine blue stain, alloxan-Schiff reaction, Mallory¢¥s aniline blue stain, Lillie¢¥s modification of Bielschowsky method and hematoxylin-eosin stain.
The results of the experiment were summarized as follows:
1) PAS reactions of basement membrane and lamina propria increased after 3 and 5 days of methionine administration.
2) Metachromasia of stratum spinosum increased after 3 days of methionine administration.
3) Alloxan-Schiff reactions of stratum granulosum and stratum spinosum increased after 7 days of methionine administration.
4) In the lamina propria, aniline blue staining of collagenous fibers increased after 7, 10 and 14 days of methionine administration.

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